质粒名称: | pLenti CMV V5-LUC Blast (w567-1) |
---|---|
出品公司: | Addgene |
目录编号: | 21474 |
存储实验室: | Eric Campeau |
载体骨架基本信息 |
|
载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体出品公司: | Clontech |
空载体大小: | 7687 bp |
完整质粒大小: | 9473 bp |
载体类型: | 哺乳细胞表达、Lentiviral, Luciferase |
筛选标记: | Blasticidin |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | Stbl3 |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | V5-LUC、Firefly Luciferase |
物种来源: | Firefly |
插入大小: | 1776 bp |
启动子: | CMV |
标签/融合蛋白: | V5 (N terminal on insert) |
克隆方法: | 限制性内切酶 |
5’克隆位点: | Bam HI (not destroyed) |
3’克隆位点: | Xba I (not destroyed) |
5’测序引物 | CMVforw |
3’测序引物 | n/a |
文献引用方法 |
|
材料和方法部分: | pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid # 21474 ; http://n2t.net/addgene:21474 ; RRID:Addgene_21474) |
参考文献部分: | A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
pLenti CMV V5-LUC Blast (w567-1)载体质粒基本信息
pLenti CMV V5-LUC Blast (w567-1)质粒图谱载体图谱和pLenti CMV V5-LUC Blast (w567-1)载体序列质粒序列多克隆位点信息
pLenti CMV V5-LUC Blast (w567-1)质粒载体简介
pLenti CMV V5-LUC Blast (w567-1)质粒序列载体序列
LOCUS pLenti CMV V5-LUC Blast (w567-1) 9473 bp DNA circular SYN 25-AUG-2024 DEFINITION 3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9473) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9473) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One."; date: "2009-08-6"; volume: "4(8)"; pages: "e6529" FEATURES Location/Qualifiers source 1..9473 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2219..2445 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2446..2626 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2673..2798 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3291..3524 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3709..3753 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4020..4136 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4159..4462 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4463..4666 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4617..4637 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4663..4687 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 4771..4795 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4807..4848 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" regulatory 4918..4927 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 4924..6576 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(4990..5008) /label=LucNrev /note="Firefly luciferase, reverse primer" protein_bind complement(6583..6607) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6638..7226 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6691..6711) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7109..7120) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7277..7297) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7282..7611 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7462..7597 /label=SV40 ori /note="SV40 origin of replication" promoter 7659..7706 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7725..8123 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" LTR 8210..8443 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8515..8636 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8552..8571) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8606..8625 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8676..8811 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(8831..8850) /label=T7 /note="T7 promoter, forward primer" promoter complement(8832..8850) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8860..8877) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8860..8876) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8869..8891) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9018..9446 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9105..9124) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9315..9336 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg 4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta 4741 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccacc 4801 atggaaggta agcctatccc taaccctctc ctcggtctcg attctacggg atccatggga 4861 accaattcag tccctaggct tttgcaaaaa gcttggcatt ccggtactgt tggtaaagcc 4921 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 4981 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 5041 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 5101 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 5161 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 5221 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 5281 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 5341 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 5401 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 5461 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 5521 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 5581 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 5641 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 5701 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 5761 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 5821 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 5881 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 5941 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 6001 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 6061 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 6121 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 6181 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 6241 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 6301 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 6361 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 6421 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 6481 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 6541 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agacccagct ttcttgtaca 6601 aagtggttga tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat 6661 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 6721 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 6781 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 6841 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 6901 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 6961 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 7021 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 7081 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 7141 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 7201 gatctccctt tgggccgcct ccccgcctgg aattctgcag atatccggtt agtaatgagt 7261 ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 7321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 7381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 7441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 7501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 7561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 7621 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat 7681 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt 7741 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct 7801 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg 7861 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg 7921 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca 7981 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag 8041 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct 8101 ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact 8161 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 8221 gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 8281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 8341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 8401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 8461 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 8521 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 8581 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 8641 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 8701 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 8761 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 8821 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 8881 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 8941 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 9001 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 9061 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 9121 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 9181 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 9241 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 9301 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 9361 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 9421 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa ttt //
pLenti CMV V5-LUC Blast (w567-1)载体图谱质粒图谱pdf版和pLenti CMV V5-LUC Blast (w567-1)载体序列质粒序列等相关资料下载
pLenti CMV V5-LUC Blast (w567-1)质粒载体应用举例
- 上一篇:AbVec2.1-mIglc2
- 下一篇:没有了